In Vitro Cell.Dev.BioL- Plant (2011) 47:667-673 DOI 10.1 007/s 1 1 627-0 1 1 -9372-5 DEVELOPMENTAL BIOLOGY/MORPHOGENESIS Calcium and calcium ionophore A23187 induce high-frequency somatic embryogenesis in cultured tissues of Coffea canephora P ex Fr Akula Ramakrishna • Parvatam Giridhar • Gokare Aswathanarayana Ravishankar
Read MoreCalcium is crucial for plant growth and development as it exerts profound influence on various biological processes. 14 The Ca 2+ permeable channels exist in a variety of cell types as demonstrated in suspension cultures of Carrot 15 and Capsicum 16 and such channels can be activated by calcium ionophore. Calcium channel inhibitors bind to plant membranes block Ca 2+ entry into …
Read MoreQuestion. 7 answers. Nov 17, 2015. For example, secondary somatic embryogenesis has great potential for large scale micropropagation, which is …
Read MorePolyamines have been implicated in plant cell proliferation and differentiation, morphogenesis, embryogenesis, and also in senescence and stress responses [50,51]. Putrescine, a polyamine synthesized upon wounding can be use in the synthesis of GABA via putrescine catabolism [ …
Read MoreThe aim of this review is to critically analyze the role of silver nitrate (AgNO 3) in modulating plant growth and development.In recent years, basic studies on ethylene regulation opened new vistas for applied research in the area of micro-propagation, somatic embryogenesis, in vitro flowering, growth promotion, fruit ripening, and expression.
Read More12%The inter-relationship between exogenous calcium (Ca2+) during cold pretreatment and cold-enhanced somatic embryogenesis was investigated using cell suspension cultures of Astragalus adsurgens Pall. Cell suspension was obtained from embryogenic callus and could be induced to form somatic embryos in the differentiation medium. Suspension cells, after cold-treatment at 8 °C …
Read MoreSomatic embryogenesis (SE) is a prominent mode of regeneration in plants. The acquisition of SE is predominantly invoked by the oxidative stress which plays an important role in signal transduction and cellular redox. Since balanced generation of oxidants is important to cellular differentiation, modulation in cell redox could be responsive to genotypic refinement for SE.
Read Moresomatic embryogenesis… High-frequency somatic embryogenesis was achieved in Coffea canephora using calcium ionophore A23187, which influences the influx of calcium into a cell. With 100 μM calcium ionophore and 5 mM calcium, 85% and 70% of cultures produced embryogenic tissue, with 105±7 and 95±8 primary embryos from each callus mass ...
Read More12%Somatic embryogenesis, the in vitro developmental program by which somatic cells are reprogrammed to undergo cellular and molecular changes that make them competent to produce somatic embryos, has been achieved with many woody plants. The program involves the stages of competence acquisition, induction and expression of the morphogenic pathway by the …
Read MorePlant morphogenesis in vitro such as somatic embryogenesis and adventitious organogenesis has provided useful systems for physiological, biochemical, and molecular biological studies on plant development. Advantages of the in vitro systems are currently being combined with genetic techniques to generate new insights into fundamental mechanisms of plant development.
Read Moreinduction of somatic embryogenesis are highly incoherent Since the initial descriptions of in vitro somatic embryo- and largely dependent upon plant genotype/cultivar, tissue- genesis [2, 3], one important characteristic of somatic type, physiological conditions of the donor plant, and varied 2 Journal of Botany cultural regimes [6].
Read More12%Under appropriate in vivo or in vitro conditions, certain somatic plant cells have the capability to initiate embryogenic development (somatic embryogenesis). Somatic embryogenesis provides an unique experimental model to understand the molecular and cellular bases of developmental plasticity in plants. In the last few years, the application of modern experimental techniques, as well …
Read MoreCentaurium erythraea (centaury) is a traditionally used medicinal plant, with a spectrum of secondary metabolites with confirmed healing properties. Centaury is an emerging model in plant developmental biology due to its vigorous regenerative potential and great developmental plasticity when cultured in vitro. Hereby, we review nearly two decades of research on somatic embryogenesis (SE) in ...
Read MoreEnergy-dispersive X-ray fluorescence technique (EDXRF) has been extensively used to characterize trace element profiles during plant growth under stre…
Read MoreAuxin-inducible genes: Auxins have emerged as one of the potent initiators of somatic embryogenesis (Raghavan, 1997;Komamine et al., 1990) and auxin-induced growth and development involves changes in gene expression (Theologis, 1986). The plant growth regulator 2, 4-D is required to induce somatic embryogenesis in many plant species.
Read MoreMelatonin (MEL) and serotonin (SER) are important indoleamines that are involved in neural transmission in mammalian cells. They are also known to be present in various genera of plants. The role (s) of these indoleamines in plants are not well known. In this study, the effects of SER, MEL, calcium, and calcium ionophore (A23187), a calcium channel activator, on somatic …
Read MoreThe calcium contained in the culture media used for Hevea somatic embryogenesis is in the form of calcium chloride (CaCl2). Increasing the CaCl2 content from 3 to 12 mM stimulated the formation of friable calli which lost their ability to produce somatic embryos (Table 1). This effect was not due to the chlorine in the CaCl2
Read MoreThe plant hormone indole-3-acetic acid (IAA) is at the center of many key morphogenic events in plants, such as embryogenesis, organogenesis, root elongation, and growth responses to environmental stimuli . Auxin is actively pumped from cell to cell by …
Read MoreA number of medium constituents were evaluated in an attempt to improve somatic embryo production in Medicago arborea ssp. arborea, using cotyledons, petioles and leaves as explants. Two culture steps were applied: in the first stage (2 months), Murashige–Skoog (MS) medium was used, containing 2,4 dichlorophenoxyacetic acid (9μM 2,4-D) and kinetin (9 μM KIN) together with different ...
Read MoreThe usage of Humulus lupulus for brewing increased the demand for high-quality plant material. Simultaneously, hop has been used in traditional medicine and recently recognized with anticancer and anti-infective properties. Tissue culture techniques have been reported for a wide range of species, and open the prospect for propagation of disease-free, genetically uniform and massive amounts of ...
Read MoreIn the present report we determine that inhibitors of Ca2+-channels (LaCl3, verapamil, niflumic acid) certainly lowered the quantity of somatic embryos in the 2c3 cell culture. This is the evidence of the influence of calcium-dependent signal system on plant embryogenesis.
Read MoreKey words: Auxins, cytokinins, in vitro culture, Linum usitatissimum, somatic embryos. Abstract The effects of plant growth regulators (PGR) on calli induction, morphogenesis and somatic embryogenesis of flax were studied. The organogenic and callus formation capacity were assessed for different types of source explants.
Read MoreCalcium, a ubiquitous signaling molecule involved in many signal transduction pathways in plant cells had been shown to become elevated in cellular levels in response to light, salinity, drought ...
Read MoreA central question in plant regeneration biology concerns the primary driving forces invoking the acquisition of somatic embryogenesis. Recently, the role of micronutrient boron (B) in the initiation and perpetuation of embryogenesis has drawn considerable attention within the scientific community. This interest may be due in part to the bewildering observation that the system-wide …
Read MoreHigh-frequency somatic embryogenesis was achieved in Coffea canephora using calcium ionophore A23187, which influences the influx of calcium into a cell. With 100 μM calcium ionophore and 5 mM calcium, 85% and 70% of cultures produced embryogenic tissue, with 105 ± 7 and 95 ± 8 primary embryos from each callus mass respectively.
Read MoreR.J. Rose, Y. Song, in Encyclopedia of Applied Plant Sciences (Second Edition), 2017 Abstract. Somatic embryogenesis (SE) is a process where embryos that can develop into whole plants are produced from somatic cells rather than through the fusion of gametes. Although SE can occur naturally, it is usually induced from cultured cells and tissues where hormones and the induced stress are key ...
Read MoreDIRECT SOMATIC EMBRYOGENESIS Source: Plant Signaling and Behaviour 8:6, June, 2013. 19. INDIRECT SOMATIC EMBRYOGENESIS 20. Somatic embryos Zygotic embryos 1. SEs are formed by sporophytic cells. 2. SEs store less amount of embryo specific reserves. 3.
Read MoreEffect of polyamines on in vitro somatic embryogenesis in Momordica charantia L. Plant Cell Tiss. Org. Cult. 97: 303-311. Pedrosa, L.F. and Vasil, I.K. (1996). Optimisation of somatic embryogenesis and long term regeneration in callus cultures of Diploperennial teosinte …
Read MoreKey features: Presents the latest trends and developments of neuromediators in plants Provides in-depth coverage of plants enriched in neurotransmitters (especially serotonin, melatonin, and dopamine) and how they are used in medicine, pharmacy, and food nutrition Discusses the physiological role of the neurotransmitters (biomediators) in non-nervous systems including the analysis of effects ...
Read MorePOLITICIZE is a leading and pioneering enterprise with the most advanced international level in R&D, manufacturing and selling of large-scale crushing & screening plants,industrial milling equipments and beneficiation plants. All of our equipment have got ISO international quality system certification.
Read More
Tel: 0086-21-58386256
E-mail: [email protected]
Fax: 0086-21-58385887
Add: No. 1688, Gaoke East Road, Pudong new district, Shanghai, China.
